Ukusetyenziswa okucetywayo

Uvavanyo olukhawulezayo lokufunyaniswa komgangatho we-antibodies engathathi hlangothi kwi-SARS-CoV-2 okanye izitofu zayo zokugonya kwigazi elipheleleyo, kwiserum, okanye kwiplasma.

Ukusetyenziswa koxilongo lwe-in vitro kuphela.

Igunyazisiwe iziqinisekiso

1. CE Ivunyiwe

2. Uluhlu olumhlophe lwaseTshayina olwamkelwe umenzi wezixhobo zovavanyo lwe-COVID 19 kunye noVavanyo oluKhawulezayo lwe-SARS-CoV-2 lwe-Neutralizing Antibody Rapid (COVID-19 Ab)

Iimbonakalo

A. Uvavanyo lwegazi, iFingerstick igazi elipheleleyo liyasebenza.

B. I-Cutoff yi-50ng / mL

C. Kulula ukusebenza, akukho zixhobo ezongezelelweyo ezifunekayo ukuqhuba uvavanyo

D. Umboniso omncinci uyafuneka.I-10ul ye-serum, i-plasma okanye i-20ul yegazi elipheleleyo yanele.

UvavanyoPiinkqubo

Vumela isixhobo sovavanyo, umfuziselo, isithinteli, kunye/okanye ulawulo lulingane neqondo lobushushu begumbi (15-30°C) phambi kovavanyo.

1) Zisa isingxobo kwiqondo lobushushu begumbi phambi kokuvula.Susa isixhobo sovavanyo kwisingxobo esivaliweyo kwaye usisebenzise ngokukhawuleza.

2) Beka isixhobo sovavanyo kwindawo ecocekileyo nethe tye.

NgeSerum okanye iMifanekiso yePlasma:

Bamba idropha ngokuthe nkqo, zoba isampuli ukuya kumgca wokuZalisa (malunga ne-10 μL), kwaye ugqithisele umfuziselo kumzekelo wequla (S) lesixhobo sovavanyo, emva koko wongeze amathontsi ama-3 e-buffer (malunga ne-120 mL) kwaye uqalise isibali-xesha. .Jonga umfanekiso ongezantsi.Kuphephe ukubambisa amaqamza omoya kwiqula elingumzekelo (S).

NgeGazi Elipheleleyo (I-Venipuncture/Fingerstick) iisampulu:

Ukusebenzisa idropha: Bamba idropha ngokuthe nkqo, zoba umfuziselo we-0.5-1 cm ngaphezulu koMda wokuZalisa, kwaye udlulisele amathontsi e-2 egazi elipheleleyo (malunga ne-20 µL) kumzekelo wequla (S) lesixhobo sovavanyo, emva koko wongeze amathontsi ama-2. yebuffer (malunga nama-90 uL) kwaye uqalise isibali-xesha.Jonga umfanekiso ongezantsi.

Ukusebenzisa i-micropipette: Umbhobho kwaye ukhuphe i-20 µL yegazi elipheleleyo kwisampulu yequla (S) lesixhobo sovavanyo, emva koko wongeze amathontsi ama-3 e-buffer (malunga ne-120 µL) kwaye uqalise isibali-xesha.Jonga umfanekiso ongezantsi.

3) Lindela ukuba umgca onemibala ubonakale.Funda iziphumo ngemizuzu eyi-10.Musa ukutolika umphumo emva kwemizuzu eli-15.

-Okuhle (+): Umgca we-C kuphela ovelayo, okanye umgca we-T ulingana nomgca we-C okanye ubuthathaka kunomgca we-C.Ibonisa ukuba kukho ii-antibodies ze-SARS-CoV-2 ezingathathi hlangothi kumzekelo.

-Engalunganga (-): Zombini umgca we-T kunye no-C uvela, xa ubukhulu bomgca we-T bunamandla kunomgca we-C.Ibonisa ukuba akukho zithinteli-ntsholongwane ze-SARS-CoV-2 ezingathathi hlangothi kwisampulu, okanye ngenye indlela i-SARS-CoV-2 i-antibody engathathi hlangothi ikwinqanaba eliphantsi kakhulu.

- Ayisebenzi: Umgca wolawulo uyasilela ukuvela.Umthamo ongonelanga wesampulu okanye iindlela ezingalunganga zenkqubo zezona zininzi